Azo dye method for demonstration of leucocyte acid phosphatase activity on blood smears.

in 60 minutes after the smear preparation . 4) Expose the smears to air for 1 hour to remove formalin. 5) Prepare the incubating solution as follows . Fifty mg of a-naphthylphosphate, 50 mg of Fast-Garnet-GBC salt, 50 ml of acetate buffer (pH 5.0, 0.1 M) are mixed, shaken well , and filtrated. 6) Incubate in it for 90 minutes at 37•Ž. 7) Wash well in distilled water . 8) Counterstain nuclei with Meyer's hematoxylin for 30 minutes. 9) Wash for 5 minutes in tap-water.